Preparation of mother culture

Mother culture is prepared by using the king’s B medium

Peptone                    : 20.0 g
K2HPO4                   : 1.5 g
Mg SO4                     : 1.5 g
Glycerol                     : 10 ml
Distilled water          : 1000 ml

The above broth is dispersed into conical flasks and autoclaved at 15 lb pressure for 15 minutes and cooled and inoculated with a loop of  P.fluorescens and incubated for 2 days.

Mass multiplication:

            The kings B medium is prepared and poured into the fermentor and sterilized at 15 lb pressure for 15 minutes. After the broth has cooled below the mother culture of P.fluorescens is added to the king’s B medium in the fermentor at the rate of 3 lit for 40 lit of the broth. Then it is incubated in the fermentor for 2 days with frequent mixing of the broth by operating the stirrer. Then the broth containing the bacterial growth is collected in plastic buckets and used for mixing with talc powder for commercial formulation.